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Case 143. HPV-16 infection (severe dysplasia) (38 y-o F)
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| Cervical smear and biopsy from a sexually active woman with colposcopic abnormality |
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Key words :
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human papillomavirus, severe dysplasia, cervical intraepithelial neoplasia-3 | ||||
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sexually transmitted infection
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| Clusters of dyskaryotic cells of parabasal type are observed in a Papanicolaou-stained preparation. | Restaining of the bleached pap smear (in situ hybridization for HPV, type 16 genome) shows dot-like reactivity in the nucleus of some dyskaryotic cells. | |
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| Cervical biopsy discloses dysplastic squamous mucosa with mild and focal surface differentiation (HE). | A few superficially located atypical cells reveal dot-like signals for HPV, type 16 genome in the nucleus (non-isotopic in situ hybridization). | |
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| HPV, type 16 genome-positive location in severe dysplasia ultrastructurally corresponds to part of chromatin, indicative of the integration of the oncogenic viral DNA into host cell DNA (pre-embedding method for in situ hybridization using a paraffin section). | ||
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